![]() ![]() From the tidbits that leaked online we know indeed that the upcoming GI Joe Rise of Cobra movie is a kind of Origins story for Cobra Commander.Īt the beginning of the GI Joe movie Cobra Commander (Joseph Gordon-Levitt) is actually called the Doctor. I guess it’s good news because the first movie is actually setting things for something much better ahead. Director Stephen Sommers composedly announced that there will be a movie sequel to GI joe during a conference in Australia. ![]()
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![]() ![]() Subsequent phenotypic screening of CL1-bearing isolates demonstrated that ~90% were resistant to QAC detergents, with in vitro experiments demonstrating that QACs could solely select for the transfer of clinical antibiotic resistance genes to a naive Escherichia coli recipient. Chemical data analysis indicated high levels of boron (a detergent marker) downstream of the WWTP. Quaternary ammonium compound (QAC) resistance genes were observed across 75% of sequenced CL1 gene cassette arrays. Over half of sequenced CL1s lacked the 3′-conserved sequence ('atypical’ CL1s) surprisingly, bacteria carrying atypical CL1s were on average resistant to more antibiotics than bacteria carrying 3′-CS CL1s. Characterisation of CL1-bearing isolates revealed that CL1s were distributed across a diverse range of bacteria, with identical complex genetic resistance determinants isolated from both human-associated and common environmental bacteria across connected sites. Using sample sites from upstream and downstream of a WWTP, we demonstrate through isolation and culture-independent analysis that WWTP effluent significantly increases both CL1 abundance and antibiotic resistance in the riverine environment. The aim of this study was to characterise the role of waste water treatment plants (WWTPs) in the dissemination of class 1 integrons (CL1s) in the riverine environment. Anthropogenic inputs increase levels of antimicrobial resistance (AMR) in the environment, however, it is unknown how these inputs create this observed increase, and if anthropogenic sources impact AMR in environmental bacteria. ![]() |
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January 2023
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